RNA Expression Data and Methods

This page provides information about the data and methods used to generate the RNA overall and differential expression evidence in Agora.

Gene-specific RNA overall and differential expression evidence can be viewed on the Evidence/RNA tab of the gene-specific pages in Agora. You can navigate to a gene’s page using search, from the Nominated Targets list, or from the Gene Comparison Tool. You can also compare RNA differential expression evidence across multiple genes using Agora’s Gene Comparison Tool.

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Data and Methods

RNA-seq data used for these analyses was generated from over 2100 samples of post-mortem brains from over 1100 individuals. In total there are nine distinct brain regions from three human cohort studies:

RNA-seq data from these studies was processed through a harmonized computational pipeline to reveal overall expression levels and to measure differential expression between AD cases and controls. The gene coexpression network is based on the original harmonized computational pipeline.

The data and methodology is publicly available through these links:

• Extended RNA-seq differential expression summary statistics

• Original RNA-seq differential expression summary statistics

• RNA-seq network coexpression analysis

Statistical Models

Differential RNA expression data can be viewed for each of the following models: